Fractionnement des peptides

Amélioration de l’identification des protéines par LC-MS/MS dans des échantillons biologiques complexes tels que le plasma par fractionnement des peptides à pH basique.

02 sf peptide fractionation v2

BioSPE™ PepFrac est utilisé pour le fractionnement des peptides par hydrophobie à pH basique. Cette méthode de fractionnement étant orthogonale à la séparation des peptides par chromatographie liquide en phase inverse, la complexité des échantillons est réduite pour le séquençage profond du protéome et l’analyse quantitative.

Le kit BioSPE™ PepFrac est composé de :

  • Colonnes de fractionnement basées sur une résine innovante en phase inverse
  • Adaptateurs (pour la centrifugation des pointes) et/ou outils de collecte (tubes de centrifugation ou plaques de collecte). Chaque kit contient neuf outils de collecte (un pour les étapes de conditionnement/chargement/lavage et un pour chaque fraction d’élution).
  • Un protocole détaillé pour un fractionnement rapide et efficace des peptides (jusqu’à 8 fractions)
  • Une solution de triéthylamine (TEA)

Pour déterminer le format et la capacité qui répondront le mieux à vos besoins, veuillez vous référer aux tableaux de l’onglet “Formats & Capacités”.

Liste des produits

Nom de produit Prix produit Product SKU Actions panier
kit 96 plate microelution biospe pepfrac 600x600

BioSPE™ PepFrac – 96 wellplate for microelution & collection plates

96 Wellplate for peptide fractionation at basic pH - Tip-shaped wells - High Capacity - 1/pk + Collection plate - 1mL - 9/pk + Triethylamine 0.1% (200mL)

Ref. : KT-Coll-µ96W-PepFrac.H8.1
kit 96 plate microelution biospe pepfrac 600x600

BioSPE™ PepFrac – 96 wellplate for microelution & collection plates

96 Wellplate for peptide fractionation at basic pH - Tip-shaped wells - Standard Capacity - 1/pk + Collection plate - 1mL - 9/pk + Triethylamine 0.1% (200mL)

Ref. : KT-Coll-µ96W-PepFrac.S8.1
kit 96 plate 1ml biospe pepfrac 600x600

BioSPE™ PepFrac – 96 wellplate & collection plates

96 Wellplate for peptide fractionation at basic pH - 1mL wells - Standard Capacity - 1/pk + Collection plate - 1mL - 9/pk + Triethylamine 0.1% (200mL)

Ref. : KT-Coll-96W-PepFrac.S8.1
kit 96 plate 1ml biospe pepfrac 600x600

BioSPE™ PepFrac – 96 wellplate & collection plates

96 Wellplate for peptide fractionation at basic pH - 1mL wells - High Capacity - 1/pk + Collection plate - 1mL - 9/pk + Triethylamine 0.1% (200mL)

Ref. : KT-Coll-96W-PepFrac.H8.1
kit tips 200ul biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 200µL & spin adapters

Kit of tips for peptide fractionation at basic pH - 200µL - High Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.H8.200.96
kit tips 200ul biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 200µL & spin adapters

Kit of tips for peptide fractionation at basic pH - 200µL - Standard Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.S8.200.96
kit tips 10ul biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 10µL & spin adapters

Kit of tips for peptide fractionation at basic pH - 10µL - Standard Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.S8.10.96
kit tips 10ul biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 10µL & spin adapters

Kit of tips for peptide fractionation at basic pH - 10µL - High Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.H8.10.96
kit tips 10ul biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 10µL Single Cell & spin adapters

Kit of tips for peptide fractionation at basic pH in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.SC8.10.96
kit tips 1ml biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 1mL & spin adapters

Kit of tips for peptide fractionation at basic pH - 1mL - Standard Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.S8.1000.96
kit tips 1ml biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 1mL & spin adapters

Kit of tips for peptide fractionation at basic pH - 1mL - High Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.H8.1000.96
kit spin micro biospe pepfrac 600x600

BioSPE™ PepFrac – µSpin

µSpin columns for peptide fractionation at basic pH - Standard Capacity - 50/pk + collection tubes - 450/pk + Triethylamine 0.1% (100mL)

Ref. : µSpin-PepFrac.S8.50
kit spin micro biospe pepfrac 600x600

BioSPE™ PepFrac – µSpin

µSpin columns for peptide fractionation at basic pH - High Capacity - 50/pk + collection tubes - 450/pk + Triethylamine 0.1% (100mL)

Ref. : µSpin-PepFrac.H8.50
kit spin mini biospe pepfrac 600x600

BioSPE™ PepFrac – Spin

Spin columns for peptide fractionation at basic pH - Standard Capacity - 50/pk + collection tubes - 450/pk + Triethylamine 0.1% (100mL)

Ref. : Spin-PepFrac.S8.50
kit spin mini biospe pepfrac 600x600

BioSPE™ PepFrac – Spin

Spin columns for peptide fractionation at basic pH - High Capacity - 50/pk + collection tubes - 450/pk + Triethylamine 0.1% (100mL)

Ref. : Spin-PepFrac.H8.50
kit spin 15ml biospe pepfrac 600x600

BioSPE™ PepFrac – Spin 15mL

Spin columns for peptide fractionation at basic pH - Standard Capacity - 15mL - 25/pk + collection tubes - 225/pk + Triethylamine 0.1% (100mL)

Ref. : Spin15-PepFrac.S8.25
kit spin 15ml biospe pepfrac 600x600

BioSPE™ PepFrac – Spin 15mL

Spin columns for peptide fractionation at basic pH - High Capacity - 15mL - 25/pk + collection tubes - 225/pk + Triethylamine 0.1% (100mL)

Ref. : Spin15-PepFrac.H8.25
kit spin 50ml biospe pepfrac 600x600

BioSPE™ PepFrac – Spin 50mL

Spin columns for peptide fractionation at basic pH - Standard Capacity - 50mL - 25/pk + collection tubes - 225/pk + Triethylamine 0.1% (100mL)

Ref. : Spin50-PepFrac.S8.25
kit spin 50ml biospe pepfrac 600x600

BioSPE™ PepFrac – Spin 50mL

Spin columns for peptide fractionation at basic pH - High Capacity - 50mL - 25/pk + collection tubes - 225/pk + Triethylamine 0.1% (100mL)

Ref. : Spin50-PepFrac.H8.25
cartridge biospe 1ml 600x600

BioSPE™ PepFrac – Cartridges 1mL

SPE cartridges for peptide fractionation at basic pH - Standard Capacity - 1mL - 50/pk + Triethylamine 0.1% (100mL)

Ref. : CAR1-PepFrac.S8.50
cartridge biospe 1ml 600x600

BioSPE™ PepFrac – Cartridges 1mL

SPE cartridges for peptide fractionation at basic pH - High Capacity - 1mL - 50/pk + Triethylamine 0.1% (100mL)

Ref. : CAR1-PepFrac.H8.50
cartridge biospe 3ml 600x600

BioSPE™ PepFrac – Cartridges 3mL

SPE cartridges for peptide fractionation at basic pH - Standard Capacity - 3mL - 50/pk + Triethylamine 0.1% (200mL)

Ref. : CAR3-PepFrac.S8.50
cartridge biospe 3ml 600x600

BioSPE™ PepFrac – Cartridges 3mL

SPE cartridges for peptide fractionation at basic pH - High Capacity - 3mL - 50/pk + Triethylamine 0.1% (200mL)

Ref. : CAR3-PepFrac.H8.50
cartridge biospe 6ml 600x600

BioSPE™ PepFrac – Cartridges 6mL

SPE cartridges for peptide fractionation at basic pH - Standard Capacity - 6mL - 25/pk + Triethylamine 0.1% (200mL)

Ref. : CAR6-PepFrac.S8.25
cartridge biospe 6ml 600x600

BioSPE™ PepFrac – Cartridges 6mL

SPE cartridges for peptide fractionation at basic pH - 6mL - 25/pk

Ref. : CAR6-PepFrac.H8.25