Dessalage des peptides

Purification et dessalage des peptides dans les approches protéomiques ascendantes.

01 sf peptide desalting v2

BioSPE™ PurePep est utilisé pour un dessalage efficace des peptides après digestion enzymatique, avant l’analyse LC-MS/MS. Jusqu’à 97% des protéines sont identifiées après dessalage du digest HeLa sur BioSPE™PurePep, avec une bonne récupération des peptides sur toute la gamme de polarité, et une bonne répétabilité (RSD < 3%).

Pour le dessalage des peptides les plus hydrophiles, nous recommandons l’utilisation de BioSPE™ PurePep Broad, qui peut retenir efficacement les petits peptides polaires. BioSPE™ PurePep Broad est également stable dans des conditions de pH extrêmes (pH 0-14) et peut s’assécher pendant le processus SPE sans conséquences sur vos résultats.

Les kits BioSPE™ PurePep et BioSPE™ PurePep Broad sont composés de :

  • Colonnes de dessalage
  • Adaptateurs (pour la centrifugation des pointes) et/ou outils de collecte (tubes à centrifuger ou plaques de collecte). Chaque kit contient deux outils de collecte (un pour les étapes de conditionnement/chargement/lavage et un pour l’étape d’élution)
  • Un protocole détaillé pour un dessalage rapide et facile

Pour déterminer le format et la capacité qui répondront le mieux à vos besoins, veuillez vous reporter aux tableaux de l’onglet “Formats et capacités”.

Liste des produits

Nom de produit Prix produit Product SKU Actions panier
kit 96 plate 1ml collection plates x2 biospe

BioSPE™ PurePep – 96 wellplate & collection plates

96 Wellplate for peptide desalting - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-96W-PurePep.S.1
kit 96 plate 1ml collection plates x2 biospe

BioSPE™ PurePep – 96 wellplate & collection plates

96 Wellplate for peptide desalting - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-96W-PurePep.H.1
kit 96 plate 1ml collection plates x2 biospe

BioSPE™ PurePep Broad – 96 wellplate & collection plates

96 Wellplate for peptide purification on broad pH range (pH 0-14) - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-96W-PurePep-Broad.H.1
kit 96 plate 1ml collection plates x2 biospe

BioSPE™ PurePep Broad – 96 wellplate & collection plates

96 Wellplate for peptide purification on broad pH range (pH 0-14) - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-96W-PurePep-Broad.S.1
kit tips 10ul 600x600

BioSPE™ PurePep Broad – Tips 10µL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.S.10.96
kit tips 10ul 600x600

BioSPE™ PurePep Broad – Tips 10µL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 10µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.H.10.96
kit tips 10ul 600x600

BioSPE™ PurePep Broad – Tips 10µL Single Cell & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.SC.10.96
kit tips 1ml 600x600

BioSPE™ PurePep Broad – Tips 1mL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 1mL -  Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.S.1000.96
kit tips 1ml 600x600

BioSPE™ PurePep Broad – Tips 1mL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 1mL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.H.1000.96
kit tips 200ul 600x600

BioSPE™ PurePep Broad – Tips 200µL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 200µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.H.200.96
kit tips 200ul 600x600

BioSPE™ PurePep Broad – Tips 200µL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 200µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.S.200.96
kit 96 plate microelution collection plates x2 biospe 600x600

BioSPE™ PurePep – 96 wellplate for microelution & collection plates

96 Wellplate for peptide desalting - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-µ96W-PurePep.H.1
kit 96 plate microelution collection plates x2 biospe 600x600

BioSPE™ PurePep – 96 wellplate for microelution & collection plates

96 Wellplate for peptide desalting - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-µ96W-PurePep.S.1
kit 96 plate microelution collection plates x2 biospe 600x600

BioSPE™ PurePep Broad – 96 wellplate for microelution & collection plates

96 Wellplate for peptide purification on broad pH range (pH 0-14) - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-µ96W-PurePep-Broad.H.1
kit 96 plate microelution collection plates x2 biospe 600x600

BioSPE™ PurePep Broad – 96 wellplate for microelution & collection plates

96 Wellplate for peptide purification on broad pH range (pH 0-14) - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-µ96W-PurePep-Broad.S.1
kit tips 10ul 600x600

BioSPE™ PurePep – Tips 10µL & spin adapters

Kit of tips for peptide desalting - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.S.10.96
kit tips 10ul 600x600

BioSPE™ PurePep – Tips 10µL & spin adapters

Kit of tips for peptide desalting - 10µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.H.10.96
kit tips 10ul 600x600

BioSPE™ PurePep – Tips 10µL Single Cell & spin adapters

Kit of tips for peptide desalting in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.SC.10.96
kit tips 1ml 600x600

BioSPE™ PurePep – Tips 1mL & spin adapters

Kit of tips for peptide desalting - 1mL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.S.1000.96
kit tips 1ml 600x600

BioSPE™ PurePep – Tips 1mL & spin adapters

Kit of tips for peptide desalting - 1mL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.H.1000.96
kit tips 200ul 600x600

BioSPE™ PurePep – Tips 200µL & spin adapters

Kit for peptide desalting containing: SPE Tips 200µL - Standard capacity - 96/pk | Spin adapters - 96/pk | 2mL collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.S.200.96
kit tips 200ul 600x600

BioSPE™ PurePep – Tips 200µL & spin adapters

Kit of tips for peptide desalting - 200µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.H.200.96
cartridge biospe 3ml 600x600

BioSPE™ PurePep Broad – Cartridges 3mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 3mL - High capacity - 50/pk

Ref. : CAR3-PurePep-Broad.H.50
kit spin mini 600x600

BioSPE™ PurePep – Spin

Spin columns for peptide desalting - Standard capacity - 50/pk + collection tubes - 100/pk

Ref. : Spin-PurePep.S.50
cartridge biospe 3ml 600x600

BioSPE™ PurePep Broad – Cartridges 3mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 3mL - Standard capacity - 50/pk

Ref. : CAR3-PurePep-Broad.S.50
cartridge biospe 6ml 600x600

BioSPE™ PurePep Broad – Cartridges 6mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 6mL - High capacity - 50/pk

Ref. : CAR6-PurePep-Broad.H.50
cartridge biospe 6ml 600x600

BioSPE™ PurePep Broad – Cartridges 6mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 6mL - Standard capacity - 50/pk

Ref. : CAR6-PurePep-Broad.S.50
kit spin mini 600x600

BioSPE™ PurePep Broad – Spin

Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50/pk + collection tubes - 100/pk

Ref. : Spin-PurePep-Broad.S.50
kit spin mini 600x600

BioSPE™ PurePep Broad – Spin

Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50/pk + collection tubes - 100/pk

Ref. : Spin-PurePep-Broad.H.50
kit spin 15ml 600x600

BioSPE™ PurePep Broad – Spin 15mL

Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 15mL - 25/pk + collection tubes - 50/pk

Ref. : Spin15-PurePep-Broad.S.25
kit spin 15ml 600x600

BioSPE™ PurePep Broad – Spin 15mL

Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 15mL - 25/pk + collection tubes - 50/pk

Ref. : Spin15-PurePep-Broad.H.25
kit spin 50ml 600x600

BioSPE™ PurePep Broad – Spin 50mL

Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50mL - 25/pk + collection tubes - 50/pk

Ref. : Spin50-PurePep-Broad.H.25
kit spin 50ml 600x600

BioSPE™ PurePep Broad – Spin 50mL

Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50mL - 25/pk + collection tubes - 50/pk

Ref. : Spin50-PurePep-Broad.S.25
kit spin micro 600x600

BioSPE™ PurePep Broad – µSpin

µSpin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50/pk + collection tubes - 100/pk

Ref. : µSpin-PurePep-Broad.S.50
kit spin micro 600x600

BioSPE™ PurePep Broad – µSpin

µSpin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50/pk + collection tubes - 100/pk

Ref. : µSpin-PurePep-Broad.H.50
kit spin micro 600x600

BioSPE™ PurePep – µSpin

µSpin columns for peptide desalting - Standard capacity - 50/pk + collection tubes - 100/pk

Ref. : µSpin-PurePep.S.50
cartridge biospe 1ml 600x600

BioSPE™ PurePep – Cartridges 1mL

SPE cartridges for peptide desalting - 1mL - Standard capacity - 50/pk

Ref. : CAR1-PurePep.S.50
cartridge biospe 1ml 600x600

BioSPE™ PurePep – Cartridges 1mL

SPE cartridges for peptide desalting - 1mL - High capacity - 50/pk

Ref. : CAR1-PurePep.H.50
cartridge biospe 3ml 600x600

BioSPE™ PurePep – Cartridges 3mL

SPE cartridges for peptide desalting - 3mL - Standard capacity - 50/pk

Ref. : CAR3-PurePep.S.50
cartridge biospe 3ml 600x600

BioSPE™ PurePep – Cartridges 3mL

SPE cartridges for peptide desalting - 3mL - High capacity - 50/pk

Ref. : CAR3-PurePep.H.50
cartridge biospe 6ml 600x600

BioSPE™ PurePep – Cartridges 6mL

SPE cartridges for peptide desalting - 6mL - Standard capacity - 50/pk

Ref. : CAR6-PurePep.S.50
cartridge biospe 6ml 600x600

BioSPE™ PurePep – Cartridges 6mL

SPE cartridges for peptide desalting - 6mL - High capacity - 50/pk

Ref. : CAR6-PurePep.H.50
kit spin mini 600x600

BioSPE™ PurePep – Spin

Spin columns for peptide desalting - High capacity - 50/pk + collection tubes - 100/pk

Ref. : Spin-PurePep.H.50
kit spin 15ml 600x600

BioSPE™ PurePep – Spin 15mL

Spin columns for peptide desalting - 15mL - High capacity - 25/pk + collection tubes - 50/pk

Ref. : Spin15-PurePep.H.25
kit spin 15ml 600x600

BioSPE™ PurePep – Spin 15mL

Spin columns for peptide desalting - 15mL - Standard capacity - 25/pk + collection tubes - 50/pk

Ref. : Spin15-PurePep.S.25
kit spin 50ml 600x600

BioSPE™ PurePep – Spin 50mL

Spin columns for peptide desalting - 50mL - High capacity - 25/pk + collection tubes - 50/pk

Ref. : Spin50-PurePep.H.25
kit spin 50ml 600x600

BioSPE™ PurePep – Spin 50mL

Spin columns for peptide desalting - 50mL - Standard capacity - 25/pk + collection tubes - 50/pk

Ref. : Spin50-PurePep.S.25
kit spin micro 600x600

BioSPE™ PurePep – µSpin

µSpin columns for peptide desalting - High capacity - 50/pk + collection tubes - 100/pk

Ref. : µSpin-PurePep.H.50
cartridge biospe 1ml 600x600

BioSPE™ PurePep Broad – Cartridges 1mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 1mL - High capacity - 50/pk

Ref. : CAR1-PurePep-Broad.H.50
cartridge biospe 1ml 600x600

BioSPE™ PurePep Broad – Cartridges 1mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 1mL - Standard capacity - 50/pk

Ref. : CAR1-PurePep-Broad.S.50