Dessalage des peptides

Purification et dessalage des peptides dans les approches protéomiques ascendantes.

BioSPE™ PurePep est utilisé pour un dessalage efficace des peptides après digestion enzymatique, avant l’analyse LC-MS/MS. Jusqu’à 97% des protéines sont identifiées après dessalage du digest HeLa sur BioSPE™PurePep, avec une bonne récupération des peptides sur toute la gamme de polarité, et une bonne répétabilité (RSD < 3%).

Pour le dessalage des peptides les plus hydrophiles, nous recommandons l’utilisation de BioSPE™ PurePep Broad, qui peut retenir efficacement les petits peptides polaires. BioSPE™ PurePep Broad est également stable dans des conditions de pH extrêmes (pH 0-14) et peut s’assécher pendant le processus SPE sans conséquences sur vos résultats.

Les kits BioSPE™ PurePep et BioSPE™ PurePep Broad sont composés de :

Colonnes de dessalage
Adaptateurs (pour la centrifugation des pointes) et/ou outils de collecte (tubes à centrifuger ou plaques de collecte). Chaque kit contient deux outils de collecte (un pour les étapes de conditionnement/chargement/lavage et un pour l’étape d’élution)
Un protocole détaillé pour un dessalage rapide et facile

Pour déterminer le format et la capacité qui répondront le mieux à vos besoins, veuillez vous reporter aux tableaux de l’onglet « Formats et capacités ».

Liste des produits

Nom de produit	Product Description	Prix produit	Actions panier
BioSPE™ PurePep – 96 wellplate & collection plates Ref. : KT-Coll-96W-PurePep.S.1	96 Wellplate for peptide desalting - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – 96 wellplate & collection plates Ref. : KT-Coll-96W-PurePep.H.1	96 Wellplate for peptide desalting - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – 96 wellplate & collection plates Ref. : KT-Coll-96W-PurePep-Broad.H.1	96 Wellplate for peptide purification on broad pH range (pH 0-14) - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – 96 wellplate & collection plates Ref. : KT-Coll-96W-PurePep-Broad.S.1	96 Wellplate for peptide purification on broad pH range (pH 0-14) - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Tips 10µL & spin adapters Ref. : KT-Adapt-Tips-PurePep-Broad.S.10.96	Kit of tips for peptide purification on broad pH range (pH 0-14) - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Tips 10µL & spin adapters Ref. : KT-Adapt-Tips-PurePep-Broad.H.10.96	Kit of tips for peptide purification on broad pH range (pH 0-14) - 10µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Tips 10µL Single Cell & spin adapters Ref. : KT-Adapt-Tips-PurePep-Broad.SC.10.96	Kit of tips for peptide purification on broad pH range (pH 0-14) in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Tips 1mL & spin adapters Ref. : KT-Adapt-Tips-PurePep-Broad.S.1000.96	Kit of tips for peptide purification on broad pH range (pH 0-14) - 1mL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Tips 1mL & spin adapters Ref. : KT-Adapt-Tips-PurePep-Broad.H.1000.96	Kit of tips for peptide purification on broad pH range (pH 0-14) - 1mL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Tips 200µL & spin adapters Ref. : KT-Adapt-Tips-PurePep-Broad.H.200.96	Kit of tips for peptide purification on broad pH range (pH 0-14) - 200µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Tips 200µL & spin adapters Ref. : KT-Adapt-Tips-PurePep-Broad.S.200.96	Kit of tips for peptide purification on broad pH range (pH 0-14) - 200µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – 96 wellplate for microelution & collection plates Ref. : KT-Coll-µ96W-PurePep.H.1	96 Wellplate for peptide desalting - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – 96 wellplate for microelution & collection plates Ref. : KT-Coll-µ96W-PurePep.S.1	96 Wellplate for peptide desalting - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – 96 wellplate for microelution & collection plates Ref. : KT-Coll-µ96W-PurePep-Broad.H.1	96 Wellplate for peptide purification on broad pH range (pH 0-14) - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – 96 wellplate for microelution & collection plates Ref. : KT-Coll-µ96W-PurePep-Broad.S.1	96 Wellplate for peptide purification on broad pH range (pH 0-14) - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Tips 10µL & spin adapters Ref. : KT-Adapt-Tips-PurePep.S.10.96	Kit of tips for peptide desalting - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Tips 10µL & spin adapters Ref. : KT-Adapt-Tips-PurePep.H.10.96	Kit of tips for peptide desalting - 10µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Tips 10µL Single Cell & spin adapters Ref. : KT-Adapt-Tips-PurePep.SC.10.96	Kit of tips for peptide desalting in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Tips 1mL & spin adapters Ref. : KT-Adapt-Tips-PurePep.S.1000.96	Kit of tips for peptide desalting - 1mL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Tips 1mL & spin adapters Ref. : KT-Adapt-Tips-PurePep.H.1000.96	Kit of tips for peptide desalting - 1mL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Tips 200µL & spin adapters Ref. : KT-Adapt-Tips-PurePep.S.200.96	Kit for peptide desalting containing: SPE Tips 200µL - Standard capacity - 96/pk \| Spin adapters - 96/pk \| 2mL collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Tips 200µL & spin adapters Ref. : KT-Adapt-Tips-PurePep.H.200.96	Kit of tips for peptide desalting - 200µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Cartridges 3mL Ref. : CAR3-PurePep-Broad.H.50	SPE cartridges for peptide purification on broad pH range (pH 0-14) - 3mL - High capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Spin Ref. : Spin-PurePep.S.50	Spin columns for peptide desalting - Standard capacity - 50/pk + collection tubes - 100/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Cartridges 3mL Ref. : CAR3-PurePep-Broad.S.50	SPE cartridges for peptide purification on broad pH range (pH 0-14) - 3mL - Standard capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Cartridges 6mL Ref. : CAR6-PurePep-Broad.H.50	SPE cartridges for peptide purification on broad pH range (pH 0-14) - 6mL - High capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Cartridges 6mL Ref. : CAR6-PurePep-Broad.S.50	SPE cartridges for peptide purification on broad pH range (pH 0-14) - 6mL - Standard capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Spin Ref. : Spin-PurePep-Broad.S.50	Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50/pk + collection tubes - 100/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Spin Ref. : Spin-PurePep-Broad.H.50	Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50/pk + collection tubes - 100/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Spin 15mL Ref. : Spin15-PurePep-Broad.S.25	Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 15mL - 25/pk + collection tubes - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Spin 15mL Ref. : Spin15-PurePep-Broad.H.25	Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 15mL - 25/pk + collection tubes - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Spin 50mL Ref. : Spin50-PurePep-Broad.H.25	Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50mL - 25/pk + collection tubes - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Spin 50mL Ref. : Spin50-PurePep-Broad.S.25	Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50mL - 25/pk + collection tubes - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – µSpin Ref. : µSpin-PurePep-Broad.S.50	µSpin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50/pk + collection tubes - 100/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – µSpin Ref. : µSpin-PurePep-Broad.H.50	µSpin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50/pk + collection tubes - 100/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – µSpin Ref. : µSpin-PurePep.S.50	µSpin columns for peptide desalting - Standard capacity - 50/pk + collection tubes - 100/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Cartridges 1mL Ref. : CAR1-PurePep.S.50	SPE cartridges for peptide desalting - 1mL - Standard capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Cartridges 1mL Ref. : CAR1-PurePep.H.50	SPE cartridges for peptide desalting - 1mL - High capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Cartridges 3mL Ref. : CAR3-PurePep.S.50	SPE cartridges for peptide desalting - 3mL - Standard capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Cartridges 3mL Ref. : CAR3-PurePep.H.50	SPE cartridges for peptide desalting - 3mL - High capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Cartridges 6mL Ref. : CAR6-PurePep.S.50	SPE cartridges for peptide desalting - 6mL - Standard capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Cartridges 6mL Ref. : CAR6-PurePep.H.50	SPE cartridges for peptide desalting - 6mL - High capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Spin Ref. : Spin-PurePep.H.50	Spin columns for peptide desalting - High capacity - 50/pk + collection tubes - 100/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Spin 15mL Ref. : Spin15-PurePep.H.25	Spin columns for peptide desalting - 15mL - High capacity - 25/pk + collection tubes - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Spin 15mL Ref. : Spin15-PurePep.S.25	Spin columns for peptide desalting - 15mL - Standard capacity - 25/pk + collection tubes - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Spin 50mL Ref. : Spin50-PurePep.H.25	Spin columns for peptide desalting - 50mL - High capacity - 25/pk + collection tubes - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Spin 50mL Ref. : Spin50-PurePep.S.25	Spin columns for peptide desalting - 50mL - Standard capacity - 25/pk + collection tubes - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – µSpin Ref. : µSpin-PurePep.H.50	µSpin columns for peptide desalting - High capacity - 50/pk + collection tubes - 100/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Cartridges 1mL Ref. : CAR1-PurePep-Broad.H.50	SPE cartridges for peptide purification on broad pH range (pH 0-14) - 1mL - High capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Cartridges 1mL Ref. : CAR1-PurePep-Broad.S.50	SPE cartridges for peptide purification on broad pH range (pH 0-14) - 1mL - Standard capacity - 50/pk	SignIn To View	SignIn To View